Juventute The rational
Background
Silfradent® technology allows to save small faction of peripheral CD34+ cells and growth factors to be included in personalized cosmetic (mask and cream). Cells and growth factors have a limited live time, but the particular formulation in which they are embedded permit to allow to extend its biological activity in time (preserved at 4 ºC, domestic refrigerator). Live time of cell can be extended for 7 d and growth factor activity can be extended by 28 d. The know how included in JUVENTUTE mask and cream: 1) the control of the osmotic pressure of the medium 2) the presence of the saline Buffer 2) the control of the pH of the medium 3) the presence of heparin mimicking block copolymer to conjugate the growth factor and extent its live time 4) the preservation of the mask and cream in the refrigerator (4 ºC) immediately after its preparation. Growth factors are proteins involved in cellular functions in applications such as wound healing and tissue regeneration. The possibility of using those growth factors and CD34+ after an aesthetic intervention with CGF induce additional benefits. |
JUVENTUTE® mask, cream and lotion, physic-chemical characterization:
JUVENTUTE® mask, cream, and lotion, physic-chemical characterization:
Juventute® Mask and Cream maintain similar characteristic than blood, to support the stabilities of growth factors and cells.
JUVENTUTE® mask, cream, and lotion, physic-chemical characterization:
Juventute® Mask and Cream maintain similar characteristic than blood, to support the stabilities of growth factors and cells.
JUVENTUTE® mask and cream: Stabilities of growth factors and cells CD34+ during storage.
Percent of recovery of CD34+ and FGF2 in Mask and Cream Juventute® stored at 4 ºC.
Percent of recovery of CD34+ and FGF2 in Mask and Cream Juventute® stored at 4 ºC.
Legend: Starting concentration: Cell CD34+ (10 x 105 / 1 mL); Fibroblast growth factor 0.03 ng/mL. Cell / CGF products were prepared by Silfradent Medifuge® procedure, from 14 apparently normal subjects. CD34+ cell products were stored at 2-8°C and analysed at time 0 (fresh products), 24 h, 7 d, 14 d and 28 days. Product viability, absolute number of viable CD34+ cells and apoptosis were determined by flow cytometry. A receptor-based enzyme-linked immunosorbant assay (ELISA) was used to assay FGF 2 concentration.
Stabilities studies allow to recommend the used of the Juventute® Mask during the first 7 d after the procedure and the cream during 28 d.
JUVENTUTE® mask and cream, directions:
The Mask Juventute – (8 g of mask + 3 mL PRP) plays a strong anti-aging action, scavenging free radicals, accumulation of toxic products of the skin and stimulating the activation of antioxidant skin systems. After making a good facial cleansing, apply a thin layer of product. After 10-15 min remove very well with warm water, then apply the cold-toning lotion with Juventute. Before applying any other product wait at least 20 min. The custom mask from professional should be stored at 4 ° - 8 ° C. It is stable for about a month and should be used within 28 days.
The Cream Juventute (30 g + 1 mL CGF CD34+ and 1 mL PRP) play an effective nourishing and regenerating due to the combination of autologous growth factors with valuable and beneficial natural substances. After cleaning your face very well, apply with light circular massage a thin layer of product, twice a day. After 15-20 min, apply the toning lotion Juventute. Do not apply additional material simultaneously. The cream customized by the trader must be stored at 4 ° - 8 °C. It is stable for about a month and should be used within 28 days.
Stabilities studies allow to recommend the used of the Juventute® Mask during the first 7 d after the procedure and the cream during 28 d.
JUVENTUTE® mask and cream, directions:
The Mask Juventute – (8 g of mask + 3 mL PRP) plays a strong anti-aging action, scavenging free radicals, accumulation of toxic products of the skin and stimulating the activation of antioxidant skin systems. After making a good facial cleansing, apply a thin layer of product. After 10-15 min remove very well with warm water, then apply the cold-toning lotion with Juventute. Before applying any other product wait at least 20 min. The custom mask from professional should be stored at 4 ° - 8 ° C. It is stable for about a month and should be used within 28 days.
The Cream Juventute (30 g + 1 mL CGF CD34+ and 1 mL PRP) play an effective nourishing and regenerating due to the combination of autologous growth factors with valuable and beneficial natural substances. After cleaning your face very well, apply with light circular massage a thin layer of product, twice a day. After 15-20 min, apply the toning lotion Juventute. Do not apply additional material simultaneously. The cream customized by the trader must be stored at 4 ° - 8 °C. It is stable for about a month and should be used within 28 days.